Cavalieri S J, Snyder I S
Infect Immun. 1982 Sep;37(3):966-74. doi: 10.1128/iai.37.3.966-974.1982.
To gain further evidence for the role of the Escherichia coli alpha-hemolysin in pathogenesis, its in vitro effects on human peripheral leukocyte function were studied. Leukocytes exposed to low doses of alpha-hemolysin responded with a marked chemiluminescence response, indicating activation of oxidative metabolism. This response was time and dose dependent. Pretreatment of leukocytes with doses of alpha-hemolysin at which nearly 80% of the cells survived decreased the ability of the cells to phagocytize bacteria and particles and to undergo chemotaxis. Premature activation of leukocytes and inhibition of phagocytosis and chemotaxis by alpha-hemolysin, if they occur in vivo, would greatly enhance the survival of an invading E. coli strain.
为了进一步获得大肠杆菌α-溶血素在发病机制中作用的证据，研究了其对人外周血白细胞功能的体外影响。暴露于低剂量α-溶血素的白细胞会产生显著的化学发光反应，表明氧化代谢被激活。这种反应具有时间和剂量依赖性。用能使近80%细胞存活的α-溶血素剂量预处理白细胞，会降低细胞吞噬细菌和颗粒以及进行趋化作用的能力。如果α-溶血素在体内导致白细胞过早激活以及抑制吞噬作用和趋化作用，将极大地提高入侵大肠杆菌菌株的存活率。
