Mangan D F, Snyder I S
Infect Immun. 1979 Dec;26(3):1014-9. doi: 10.1128/iai.26.3.1014-1019.1979.
Escherichia coli organisms with mannose-sensitive adherence factors (adhesins) are known to associate with human peripheral leukocytes (WBCs) in vitro in the absence of serum. To determine whether the WBC respiratory burst is activated during the interaction with E. coli, WBC chemiluminescence was measured. E. coli with mannose-sensitive adhesins stimulated a sharp burst of chemiluminescence which peaked 15 to 30 min after the bacteria and WBCs were mixed. Stimulation of chemiluminescence could be abrogated by including 10 mM alpha-methyl-D-mannoside in the test suspension. The addition of alpha-methyl-D-mannoside up to 20 min after the E. coli and WBCs were combined caused a rapid decrease in chemiluminescence. E. coli stimulation of chemiluminescence could not be inhibited by pretreating the WBCs with purified type 1 pili (fimbriae). E. coli lacking mannose-sensitive adhesins failed to stimulate chemiluminescence. The results emphasize the importance of mannose-sensitive adhesins in the association of E. coli with WBCs and suggest that the E. coli-WBC interaction system may be a useful tool for studying the mechanisms involved in the activation of the respiratory burst during phagocytosis.
已知带有甘露糖敏感黏附因子（黏附素）的大肠杆菌在无血清条件下可在体外与人外周血白细胞（WBC）发生关联。为确定在与大肠杆菌相互作用期间白细胞呼吸爆发是否被激活，对白细胞化学发光进行了测定。带有甘露糖敏感黏附素的大肠杆菌刺激了化学发光的急剧爆发，在细菌与白细胞混合后15至30分钟达到峰值。在测试悬液中加入10 mM α-甲基-D-甘露糖苷可消除化学发光的刺激。在大肠杆菌与白细胞混合后长达20分钟加入α-甲基-D-甘露糖苷会导致化学发光迅速下降。用纯化的1型菌毛（菌毛）预处理白细胞不能抑制大肠杆菌对化学发光的刺激。缺乏甘露糖敏感黏附素的大肠杆菌无法刺激化学发光。这些结果强调了甘露糖敏感黏附素在大肠杆菌与白细胞关联中的重要性，并表明大肠杆菌-白细胞相互作用系统可能是研究吞噬作用期间呼吸爆发激活所涉及机制的有用工具。
