对由IncB质粒R864a所指定的引发酶的检测。
Detection of primase specified by IncB plasmid R864a.
作者信息
Dalrymple B P, Williams P H
出版信息
J Bacteriol. 1982 Nov;152(2):901-3. doi: 10.1128/jb.152.2.901-903.1982.
Abstract
Plasmid R864a (IncB) contains nucleotide sequences homologous with the sog primase determinant of IncI alpha plasmid ColIb-P9. Extracts of Escherichia coli carrying a mutant R864a derepressed for transfer functions showed enhanced primase activity, and contained a large polypeptide identical in size (apparent Mr = 220,000) to the IncI alpha sog gene product.
摘要
质粒R864a（IncB）含有与IncIα质粒ColIb - P9的sog引发酶决定簇同源的核苷酸序列。携带转移功能去阻遏的突变型R864a的大肠杆菌提取物显示出增强的引发酶活性，并且含有一种大小（表观Mr = 220,000）与IncIα sog基因产物相同的大分子量多肽。
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