多种嗜肺军团菌胞外类胰凝乳蛋白酶活性的证明。
Demonstration of extracellular chymotrypsin-like activity from various Legionella species.
作者信息
Berdal B P, Olsvik O, Myhre S, Omland T
出版信息
J Clin Microbiol. 1982 Sep;16(3):452-7. doi: 10.1128/jcm.16.3.452-457.1982.
Abstract
Concentrated extracellular supernatants from Legionella pneumophila, L. bozemanii, L. dumoffi, L. gormanii, but not L. micdadei, exhibited a strong chymotrypsin-like activity upon synthetic chromogenic tri- and tetrapeptides. Bacterial cell sonic extracts showed low protease activities, different from those of the extracellular concentrates. Extracellular concentrates were also tested in the API ZYM system, where weak protease activity only was recorded. Protease inhibitors decreased the activity of the extracellular Legionella proteases upon the chromogenic peptides.
摘要
嗜肺军团菌、博兹曼军团菌、杜莫夫军团菌、戈尔曼军团菌的浓缩细胞外上清液（而米克戴德军团菌的上清液未表现出此特性）对合成生色三肽和四肽表现出较强的类胰凝乳蛋白酶活性。细菌细胞超声提取物显示出较低的蛋白酶活性，这与细胞外浓缩物的活性不同。细胞外浓缩物也在API ZYM系统中进行了测试，结果仅记录到微弱的蛋白酶活性。蛋白酶抑制剂降低了细胞外军团菌蛋白酶对生色肽的活性。
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