活性炭酵母浸出液琼脂：嗜肺军团菌的初次分离培养基。
Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila.
作者信息
Feeley J C, Gibson R J, Gorman G W, Langford N C, Rasheed J K, Mackel D C, Baine W B
出版信息
J Clin Microbiol. 1979 Oct;10(4):437-41. doi: 10.1128/jcm.10.4.437-441.1979.
Abstract
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% (wt/vol). Comparison of charcoal-yeast extract and F-G agars showed that a greater number of colony-forming units of L. pneumophila was recovered from a standardized tissue inoculum on charcoal-yeast extract agar (4.35 x 10(6) colony-forming units) than on F-G agar (4.85 x 10(4) colony-forming units). Macroscopic colonies of L. pneumophila were visible on the new medium within 3 days, whereas 4 days of growth was required on F-G agar.
摘要
活性炭酵母浸出物琼脂是一种新型细菌培养基，能很好地支持嗜肺军团菌生长。它是对现有嗜肺军团菌培养基F-G琼脂进行改良后得到的。用酵母浸出物替代酪蛋白酸水解物作为蛋白质来源。不添加牛肉浸出物和淀粉。加入0.20%（重量/体积）的活性炭（诺里特A或诺里特SG）。活性炭酵母浸出物琼脂和F-G琼脂的比较表明，从标准化组织接种物中在活性炭酵母浸出物琼脂上回收的嗜肺军团菌菌落形成单位数量（4.35×10⁶个菌落形成单位）比在F-G琼脂上（4.85×10⁴个菌落形成单位）更多。在新培养基上3天内就能看到嗜肺军团菌的宏观菌落，而在F-G琼脂上则需要4天生长时间。
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