Ylikorkala O, Huttunen K, Järvi J, Viinikka L
Clin Nephrol. 1982 Aug;18(2):83-7.
To study the effects of uremia and hemodialysis on the production rates of antiaggregatory prostacyclin (PGI2) and proaggregatory thromboxane A2 (TxA2), we collected serial plasma samples from eight patients with chronic uremia before, during and after hemodialysis and assayed them for 6-keto-PGF1 alpha and TxB2, the stable metabolites of PGI2 and TxA2, respectively. In addition, the capacity of the platelets to produce TxB2 during spontaneous clotting was studied by measuring the TxB2 levels in serum incubated at +37 degrees C for 60 minutes. The PGI2 production of the uremia patients before hemodialysis was less (P less than 0.001) than that of healthy volunteers. It rose significantly following heparinization and remained elevated during hemodialysis. TxB2 generation by platelets during clotting was diminished in uremia. Plasma TxB2 levels were normal before, but increased during hemodialysis. Thus, profound changes in the PGI2/TxA2-system seem to be associated with uremia and hemodialysis.
为研究尿毒症及血液透析对抗聚集性前列环素（PGI2）和促聚集性血栓素A2（TxA2）生成率的影响，我们收集了8例慢性尿毒症患者在血液透析前、透析期间及透析后的系列血浆样本，并分别检测其中PGI2和TxA2的稳定代谢产物6-酮-PGF1α和TxB2。此外，通过测量37℃孵育60分钟的血清中TxB2水平，研究了血小板在自发凝血过程中产生TxB2的能力。尿毒症患者血液透析前的PGI2生成量低于健康志愿者（P＜0.001）。肝素化后PGI2生成量显著增加，并在血液透析期间持续升高。尿毒症患者凝血过程中血小板产生的TxB2减少。血浆TxB2水平在透析前正常，但在透析期间升高。因此，PGI2/TxA2系统的深刻变化似乎与尿毒症及血液透析有关。
