Verney C, Grzanna R, Farkas E
Dev Neurosci. 1982;5(4):369-74. doi: 10.1159/000112788.
Postnatal ontogeny of dopamine-beta-hydroxylase (DBH)-like immunoreactive fibers in the rat cerebellar cortex has been studied by immunofluorescence on sagittal sections of the vermis. Except in the case of unusual thick highly fluorescent fibers noticed in the external granular layer during the first 2 weeks after birth, the arrival of DBH-containing fibers through the cerebellar cortex follows an inside-out gradient: at birth sparse fluorescent axons are observed in the album of the cerebellum. They invade the internal granular layer at postnatal day 2 (P2) and reach the Purkinje cell layer at P5. From P5 to P15, the density of fibers increases in this layer, which becomes the most innervated layer of the cerebellar cortex, as in the adult. When the external grains migrate, DBH-containing fibers extend progressively from depth to surface in the molecular layer and show an adult pattern at P30. The relationship between our results and other morphological and biochemical studies is discussed.
通过对大鼠小脑蚓部矢状切片进行免疫荧光研究，探讨了大鼠小脑皮质中多巴胺-β-羟化酶（DBH）样免疫反应性纤维的出生后个体发生情况。除了在出生后前两周的外颗粒层中发现异常粗大的高荧光纤维外，含DBH纤维通过小脑皮质的过程遵循由内向外的梯度：出生时，在小脑白质中观察到稀疏的荧光轴突。它们在出生后第2天（P2）侵入内颗粒层，并在P5到达浦肯野细胞层。从P5到P15，该层纤维密度增加，成为小脑皮质中神经支配最多的层，与成年时相同。当外颗粒迁移时，含DBH纤维在分子层中从深层逐渐向表层延伸，并在P30呈现成年模式。讨论了我们的结果与其他形态学和生化研究之间的关系。
