Häyry P, von Willebrand E, Ahonen J, Eklund B
Scand J Urol Nephrol Suppl. 1981;64:52-5.
We have performed fine-needle aspiration biopsies to well-to-do and repeatedly rejecting renal allografts. After biopsy the aspirated cells were treated either (a) with monospecific rabbit antisera to HLA-ABC, -DR or beta 2m, or (b) with ordinary anti-HLA-A and B alloantisera. After wash, the cell-bound antibody was chased with Staphylococcus aureus Cowan I bacteria and the antibody-binding cell type(s) was analyzed from stained cytocentrifuged cell smears. In normal, non-transplanted human kidneys the passenger leukocytes and (part of) the endothelial cells bound strongly both anti-HLA-ABC and -DR, the tubular cells weakly anti-ABC but not anti-DR while the glomerular cells did not bind either type of antiserum or bound them only very weakly. In 7 or 8 transplants with an excellent postoperative course we were unable to demonstrate the HLA-ABC and DR antigens on the graft endothelial component. Instead, in 7 of 8 transplants with repeated late rejection episodes, both the HLA-ABC and DR antigens were distinctly expressed on the endothelial cells of the graft.
我们对状况良好但反复出现排斥反应的肾移植受者进行了细针穿刺活检。活检后，吸出的细胞分别采用以下两种方式处理：(a) 用针对 HLA - ABC、- DR 或β2m 的单特异性兔抗血清处理；(b) 用普通的抗 HLA - A 和 B 同种异体抗血清处理。洗涤后，用金黄色葡萄球菌 Cowan I 菌追踪细胞结合的抗体，并从经染色的细胞离心涂片分析结合抗体的细胞类型。在正常的、未移植的人肾中，过客白细胞和（部分）内皮细胞强烈结合抗 HLA - ABC 和 - DR，肾小管细胞弱结合抗 ABC 但不结合抗 DR，而肾小球细胞不结合任何一种抗血清或仅非常弱地结合它们。在 7 或 8 例术后过程良好的移植病例中，我们未能在移植肾的内皮成分上检测到 HLA - ABC 和 DR 抗原。相反，在 8 例反复出现晚期排斥反应的移植病例中，有 7 例移植肾的内皮细胞上明显表达了 HLA - ABC 和 DR 抗原。
