Cripps A L, Blunden G
Steroids. 1978 May;31(5):661-9. doi: 10.1016/s0039-128x(78)80006-3.
A gas-liquid chromatographic method has been devised for the routine estimation of the hecogenin [3beta-hydroxy-(25R)-5beta-spirostan-12-one] and tigogenin [ (25R)-5beta-spirostan-3beta-ol] contents of Agave sisalana leaf and juice samples and of the crude sapogenin concentrates known as "coffee grounds". Because of partial degradation of the sapogenins in the GLC system it was found necessary to acetylate the compounds prior to their estimation. In East African samples the tigogenin proportion of the total sapogenin content is usually about 10%. At this level, the 95% inverse tolerance limits on predicted tigogenin weights are approximately +/- 7%.
已设计出一种气液色谱法，用于常规测定剑麻叶片和汁液样品以及名为“咖啡渣”的粗制皂草苷浓缩物中的海柯皂苷元[3β-羟基-(25R)-5β-螺旋甾烷-12-酮]和替告皂苷元[(25R)-5β-螺旋甾烷-3β-醇]含量。由于皂草苷在气相色谱系统中会部分降解，因此发现在测定之前有必要将这些化合物乙酰化。在东非样品中，替告皂苷元占总皂草苷含量的比例通常约为10%。在此水平下，预测替告皂苷元重量的95%反向耐受限度约为±7%。
