Kilejian A, Behr M A, Lee Y Y
J Parasitol. 1982 Dec;68(6):1021-8.
Rabbits were immunized with merozoite-enriched preparations of erythrocytic and exoerythrocytic Plasmodium lophurae. The antisera were used to compare antigens of the two types of merozoites. The indirect immunofluorescent antibody test showed the presence of common antigens. The growth of exoerythrocytic parasites was inhibited by the homologous antiserum and to a lesser extent by the antiserum prepared against erythrocytic forms. Cultures of exoerythrocytic parasites as well as their normal host cells were labeled metabolically with 35S-methionine, tritiated proline and glucosamine. Nonidet P-40 extracts of labeled merozoite-enriched preparations, infected cells, and normal cells were immunoprecipitated with the two types of antisera and the immunoprecipitates were analyzed on polyacrylamide gels. The results showed that erythrocytic and exoerythrocytic merozoites have several common proteins. A major difference was a glycoprotein with an approximate molecular weight of 110,000 daltons. This glycoprotein was associated with the surface of exoerythrocytic merozoites and was not recognized by antibodies prepared against erythrocytic forms.
用富含红细胞内期和红细胞外期约氏疟原虫裂殖子的制剂对兔子进行免疫。用这些抗血清比较两种类型裂殖子的抗原。间接免疫荧光抗体试验显示存在共同抗原。红细胞外期寄生虫的生长受到同源抗血清的抑制，而受到针对红细胞内期形式制备的抗血清的抑制程度较小。用35S-甲硫氨酸、氚标记的脯氨酸和葡糖胺对红细胞外期寄生虫及其正常宿主细胞进行代谢标记。用两种类型的抗血清对标记的富含裂殖子的制剂、感染细胞和正常细胞的Nonidet P-40提取物进行免疫沉淀，并在聚丙烯酰胺凝胶上分析免疫沉淀物。结果表明，红细胞内期和红细胞外期裂殖子有几种共同的蛋白质。一个主要差异是一种分子量约为110,000道尔顿的糖蛋白。这种糖蛋白与红细胞外期裂殖子的表面相关，并且不被针对红细胞内期形式制备的抗体识别。
