Mindich L, Bamford D, Goldthwaite C, Laverty M, Mackenzie G
J Virol. 1982 Dec;44(3):1013-20. doi: 10.1128/JVI.44.3.1013-1020.1982.
We isolated nonsense mutants of bacteriophage PRD1, a lipid-containing polyhedral virus capable of infecting many genera of gram-negative bacteria. These mutants were grouped into 19 classes on the basis of genetic complementation and sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis. PRD1 infection led to the synthesis of at least 25 viral proteins, 17 of which were components of mature virions. The synthesis of proteins fell into the following three classes: very early, middle early, and late. Two of the very early proteins, P1 and P8, had an effect on DNA synthesis, host protein synthesis shutoff, and the turning on of middle and late protein synthesis. Another very early protein, P12, was involved in the shutoff of early protein synthesis. Two genes were identified as affecting lysis of the host. One appeared to be a lysin, whereas the other was an accessory lytic factor.
我们分离出了噬菌体PRD1的无义突变体，PRD1是一种含脂质的多面体病毒，能够感染许多革兰氏阴性菌属。基于遗传互补和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，这些突变体被分为19类。PRD1感染导致至少25种病毒蛋白的合成，其中17种是成熟病毒粒子的组成成分。蛋白质的合成分为以下三类：极早期、中早期和晚期。两种极早期蛋白P1和P8对DNA合成、宿主蛋白合成的关闭以及中晚期蛋白合成的开启有影响。另一种极早期蛋白P12参与早期蛋白合成的关闭。鉴定出两个影响宿主裂解的基因。一个似乎是溶菌酶，而另一个是辅助裂解因子。
