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含脂质噬菌体PRD1的结构：盐酸胍对野生型和无义突变噬菌体颗粒的破坏作用

Structure of the lipid-containing bacteriophage PRD1: disruption of wild-type and nonsense mutant phage particles with guanidine hydrochloride.

作者信息

Bamford D, Mindich L

出版信息

J Virol. 1982 Dec;44(3):1031-8. doi: 10.1128/JVI.44.3.1031-1038.1982.

DOI:10.1128/JVI.44.3.1031-1038.1982
PMID:6816951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256363/
Abstract

The lipid-containing bacteriophage PRD1 was disrupted, and the subviral particles were studied. Guanidine treatment released two phage proteins (P3 and P5). These proteins form the polyhedral capsid. The remaining phage proteins were associated with the phage membrane vesicle. The vesicle was capable of forming a tubular structure. The isolated phage membrane vesicles aggregated readily. We found that aggregation and tube formation were associated with specific phage proteins (P11 and P18, respectively) by using protease treatment and an analysis of nonsense mutant phage particles. In addition, the possibility that free vesicles might be precursors to empty virions was studied.

摘要

含脂质的噬菌体PRD1被破坏，对亚病毒颗粒进行了研究。胍处理释放出两种噬菌体蛋白（P3和P5）。这些蛋白形成多面体衣壳。其余的噬菌体蛋白与噬菌体膜泡相关。该膜泡能够形成管状结构。分离出的噬菌体膜泡很容易聚集。通过蛋白酶处理和对无义突变噬菌体颗粒的分析，我们发现聚集和管状结构的形成分别与特定的噬菌体蛋白（P11和P18）有关。此外，还研究了游离膜泡可能是空病毒体前体的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/a6d120e1ca83/jvirol00153-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/480b4b65a8d8/jvirol00153-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/b9df78072d98/jvirol00153-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/a6d120e1ca83/jvirol00153-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/480b4b65a8d8/jvirol00153-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/b9df78072d98/jvirol00153-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1252/256363/a6d120e1ca83/jvirol00153-0280-a.jpg

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本文引用的文献

1
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
2
Comparison of the lipid-containing bacteriophages PRD1, PR3, PR4, PR5 and L17.含脂质噬菌体PRD1、PR3、PR4、PR5和L17的比较
J Gen Virol. 1981 Dec;57(Pt 2):365-73. doi: 10.1099/0022-1317-57-2-365.
3
Assembly of bacteriophage PRD1: particle formation with wild-type and mutant viruses.噬菌体PRD1的组装：野生型和突变型病毒的颗粒形成
Nanoscale. 2018 Apr 26;10(16):7769-7779. doi: 10.1039/c8nr00196k.
4
Mechanism of membranous tunnelling nanotube formation in viral genome delivery.病毒基因组传递中膜性隧道纳米管形成的机制。
PLoS Biol. 2013 Sep;11(9):e1001667. doi: 10.1371/journal.pbio.1001667. Epub 2013 Sep 24.
5
Lipids of archaeal viruses.古菌病毒的脂质。
Archaea. 2012;2012:384919. doi: 10.1155/2012/384919. Epub 2012 Sep 20.
6
Bacteriophage assembly.噬菌体组装。
Viruses. 2011 Mar;3(3):172-203. doi: 10.3390/v3030172. Epub 2011 Feb 25.
7
The closest relatives of icosahedral viruses of thermophilic bacteria are among viruses and plasmids of the halophilic archaea.嗜热细菌二十面体病毒的近亲存在于嗜盐古菌的病毒和质粒之中。
J Virol. 2009 Sep;83(18):9388-97. doi: 10.1128/JVI.00869-09. Epub 2009 Jul 8.
8
An icosahedral algal virus has a complex unique vertex decorated by a spike.一种二十面体藻类病毒有一个由刺突装饰的复杂独特顶点。
Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11085-9. doi: 10.1073/pnas.0904716106. Epub 2009 Jun 18.
9
Tale of two spikes in bacteriophage PRD1.噬菌体PRD1中的两个尖峰的故事
Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6666-71. doi: 10.1073/pnas.0608625104. Epub 2007 Apr 6.
10
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J Virol. 2007 Mar;81(6):2970-9. doi: 10.1128/JVI.02211-06. Epub 2007 Jan 3.
J Virol. 1982 Dec;44(3):1021-30. doi: 10.1128/JVI.44.3.1021-1030.1982.
4
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J Virol. 1982 Dec;44(3):1013-20. doi: 10.1128/JVI.44.3.1013-1020.1982.
5
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.聚乙二醇存在下噬菌体的快速沉降及其在大规模病毒纯化中的应用
Virology. 1970 Mar;40(3):734-44. doi: 10.1016/0042-6822(70)90218-7.
6
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.平板凝胶上噬菌体T7早期RNA和蛋白质的分析。
J Mol Biol. 1973 Sep 15;79(2):237-48. doi: 10.1016/0022-2836(73)90003-x.
7
Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage.PDR1的特性，一种依赖质粒的广泛宿主范围DNA噬菌体。
J Virol. 1974 Sep;14(3):689-99. doi: 10.1128/JVI.14.3.689-699.1974.
8
Proteins of bacteriophage phi6.噬菌体phi6的蛋白质
J Virol. 1975 Sep;16(3):685-95. doi: 10.1128/JVI.16.3.685-695.1975.
9
Isolation of nonsense suppressor mutants in Pseudomonas.假单胞菌中无义抑制突变体的分离
J Bacteriol. 1976 Apr;126(1):177-82. doi: 10.1128/jb.126.1.177-182.1976.
10
Phospholipids in an Escherichia coli bacteriophage.大肠杆菌噬菌体中的磷脂
FEBS Lett. 1975 Oct 15;58(1):43-6. doi: 10.1016/0014-5793(75)80221-3.