Gonias S L, Fuchs H E, Pizzo S V
Thromb Haemost. 1982 Oct 29;48(2):208-10.
Radiolabeled alpha 2-antiplasmin cleared slowly from the circulation of mice. Complex formation with either plasmin or trypsin resulted in a significant increase in the plasma elimination rate of the protease inhibitor. Approximately 20 min and 14 min were required for 50% of the injected alpha 2-antiplasmin-plasmin and alpha 2-antiplasmin-trypsin to clear from the circulation, respectively. Significant competition was observed when radiolabeled alpha 2-antiplasmin-plasmin was cleared in the presence of a large molar excess of unlabeled alpha 2-antiplasmin-plasmin. alpha 1-Antitrypsin-trypsin failed to complete with radiolabeled alpha 2-antiplasmin-plasmin even when present at 2000 fold molar excess. Organ distribution studies localized the major site of alpha 2-antiplasmin-plasmin clearance in the liver. Microscopic autoradiography data suggested that the cell responsible for the clearance pathway was the hepatocyte.
放射性标记的α2-抗纤溶酶从小鼠循环系统中清除缓慢。与纤溶酶或胰蛋白酶形成复合物会导致蛋白酶抑制剂的血浆清除率显著增加。分别约需20分钟和14分钟，注射的α2-抗纤溶酶-纤溶酶和α2-抗纤溶酶-胰蛋白酶的50%才能从循环中清除。当在大量摩尔过量的未标记α2-抗纤溶酶-纤溶酶存在的情况下清除放射性标记的α2-抗纤溶酶-纤溶酶时，观察到显著的竞争。即使α1-抗胰蛋白酶-胰蛋白酶以2000倍摩尔过量存在，也无法与放射性标记的α2-抗纤溶酶-纤溶酶竞争。器官分布研究确定肝脏是α2-抗纤溶酶-纤溶酶清除的主要部位。显微放射自显影数据表明，负责清除途径的细胞是肝细胞。
