Hollingdale M R, Leland P
Trans R Soc Trop Med Hyg. 1982;76(5):624-6. doi: 10.1016/0035-9203(82)90225-5.
An immunoperoxidase antibody (IPA) technique is described for the detection of exoerythrocytic (EE) stages of Plasmodium berghei in rat liver sections or cultured W138 cells. EE stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of W138 cells. By 42 hours in liver sections and 48 hours in W138 cells, EE parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within EE parasites in W138 cells, or extracellularly on adjacent cells.
描述了一种免疫过氧化物酶抗体（IPA）技术，用于检测大鼠肝脏切片或培养的W138细胞中伯氏疟原虫的红细胞外（EE）期。接种子孢子后12小时在肝脏切片中检测到EE期，但在接种W138细胞后最早3小时即可检测到。在肝脏切片中42小时、W138细胞中48小时时，低倍显微镜下即可轻松看到EE期寄生虫，在W138细胞中，接种72小时内可在EE期寄生虫内清晰看到裂殖子，或在相邻细胞外看到。
