Laurell C B, Weiber H, Ohlsson K, Rannevik G
Clin Chim Acta. 1982 Dec 9;126(2):161-70. doi: 10.1016/0009-8981(82)90032-8.
The electrophoretic seminal plasma protein pattern changes obviously within a few hours if the plasma is not frozen after the ejaculation. Mainly cathodal proteins disappear. Addition of serine protease inhibitors has no apparent affect, but o-phenanthroline markedly retards the conversions seen on electrophoresis. The subcellular organelles in seminal plasma are shown to contain a zinc ion dependent peptidase with optimal activity at pH 7.8. Estimations of enzyme activity may be performed using the elastase substrates n-tert butyloxycarbonyl-L-alanine-paranitrophenylester (Boc(Ala)) and succinyl(alanine)3-paranitroanilide (Suc(Ala)3pNA). The enzyme is inactivated by addition of o-phenanthroline and is completely reactivated by the addition of zinc ions.
如果射精后的精浆不冷冻，其电泳蛋白图谱会在数小时内明显改变。主要是阴极蛋白消失。添加丝氨酸蛋白酶抑制剂没有明显影响，但邻菲罗啉能显著延缓电泳时出现的转化。研究表明，精浆中的亚细胞器含有一种锌离子依赖性肽酶，在pH 7.8时活性最佳。可以使用弹性蛋白酶底物N-叔丁氧羰基-L-丙氨酸-对硝基苯酯（Boc(Ala)）和琥珀酰（丙氨酸）3-对硝基苯胺（Suc(Ala)3pNA）来测定酶活性。添加邻菲罗啉会使该酶失活，而添加锌离子则可使其完全重新激活。
