Crabbe M J
Comput Biol Med. 1982;12(4):263-83. doi: 10.1016/0010-4825(82)90031-2.
A basic computer program has been developed which will compute statistical parameters on initial velocity data, compute and plot mean initial velocities and 95% confidence limits in all graphical transformed spaces, calculate Km and Vmax values by weighted non-linear regression to a 1:1 rational polynomial function, and plot residual scatter and Hill plot slope from the data input. Plots and calculations for temperature dependence and reversible and irreversible inhibition can also be obtained. The program is suitable for any desk-top microcomputer.
已经开发了一个基本的计算机程序,它将对初始速度数据计算统计参数,在所有图形变换空间中计算并绘制平均初始速度和95%置信限,通过加权非线性回归到1:1有理多项式函数来计算Km和Vmax值,并根据输入数据绘制残差散点图和希尔图斜率。还可以获得温度依赖性以及可逆和不可逆抑制的图和计算结果。该程序适用于任何台式微型计算机。