Dobersen M J, Scharff J E
Diabetes. 1982 May;31(5 Pt 1):459-62. doi: 10.2337/diab.31.5.459.
Sera from patients with insulin-dependent diabetes mellitus (IDDM) containing islet cell surface antibodies (ICSA) were studied for their capacity to lyse cultured rat islet cells. The uptake of ethidium bromide was used to identify lysed cells and immunofluorescent staining with antisera to insulin, glucagon, somatostatin, or pancreatic polypeptide was used to identify the different islet cell types (B-, A-, D-, and PP-cells, respectively). Our experiments showed that in the presence of complement, sera containing ICSA lysed 81% of the B-cells, but 10% or less of the A-, D-, and PP-cells. Normal control sera resulted in lysis of less than 4% of each of the islet cell types. The demonstration that ICSA are preferentially lytic for B-cells may be important in defining the role of these autoantibodies in the pathogenesis of IDDM, particularly since the B-cell mass in diabetics is markedly reduced relative to the other islet cell types.
对含有胰岛细胞表面抗体（ICSA）的胰岛素依赖型糖尿病（IDDM）患者的血清进行了研究，以检测其裂解培养的大鼠胰岛细胞的能力。用溴化乙锭摄取法鉴定裂解细胞，并用抗胰岛素、胰高血糖素、生长抑素或胰多肽的抗血清进行免疫荧光染色，以鉴定不同类型的胰岛细胞（分别为B细胞、A细胞、D细胞和PP细胞）。我们的实验表明，在补体存在的情况下，含有ICSA的血清可裂解81%的B细胞，但A细胞、D细胞和PP细胞的裂解率不到10%。正常对照血清导致每种胰岛细胞类型的裂解率均低于4%。证明ICSA对B细胞具有优先裂解作用，这对于确定这些自身抗体在IDDM发病机制中的作用可能很重要，特别是因为糖尿病患者的B细胞数量相对于其他胰岛细胞类型明显减少。
