Julou C, Bolotin-Fukuhara M
Mol Gen Genet. 1982;188(2):256-60. doi: 10.1007/BF00332684.
Among the mitochondrial conditional mutations localized in the gene coding for the 21S ribosomal RNA, one--ts 902--produces severely reduced amounts of 21S RNA and 50S subunit. We investigated its physiological properties and found that this thermosensitive mutation was associated with highly pleiotropic effects. The mutant phenotype is associated with cell death in certain conditions, and with a massive accumulation of rho- mutants at non-permissive temperature. Furthermore, interactions with the sites of action of erythromycin and chloramphenicol, both localized within the 21S rRNA, were detected. The mutant is hypersensitive to erythromycin and has a cis-incompatibility with the chloramphenicol-resistant mutation CR321. Ts 902 thus appears to have a dual effect, not only at the ribosomal level but also at a cellular level.
在定位在编码21S核糖体RNA基因中的线粒体条件突变中，其中一个——ts 902——产生的21S RNA和50S亚基数量严重减少。我们研究了它的生理特性，发现这种温度敏感突变与高度多效性效应相关。突变表型在某些条件下与细胞死亡相关，并且在非允许温度下与rho-突变体的大量积累相关。此外，还检测到与均位于21S rRNA内的红霉素和氯霉素作用位点的相互作用。该突变体对红霉素高度敏感，并且与氯霉素抗性突变CR321存在顺式不相容性。因此，ts 902似乎具有双重作用，不仅在核糖体水平，而且在细胞水平。
