Glotz C, Zwieb C, Brimacombe R, Edwards K, Kössel H
Nucleic Acids Res. 1981 Jul 24;9(14):3287-306. doi: 10.1093/nar/9.14.3287.
Short base-paired RNA fragments, and fragments containing intra-RNA cross-links, were isolated from E. coli 23S rRNA or 50S ribosomal subunits by two-dimensional gel electrophoresis. The interactions thus found were used as a first basis for constructing a secondary structure model of the 23S rRNA. Sequence comparison with the 23S rDNA from Z. mays chloroplasts, as well as with the 16S (large subunit) rDNA from human and mouse mitochondria, enabled the experimental model to be improved and extrapolated to give complete secondary structures of all four species. The structures are organized in well-defined domains, with over 450 compensating base changes between the two 23S species. Some ribosomal structural "'switches" were found, one involving 5S rRNA.
通过二维凝胶电泳从大肠杆菌23S rRNA或50S核糖体亚基中分离出短的碱基配对RNA片段以及含有RNA内交联的片段。由此发现的相互作用被用作构建23S rRNA二级结构模型的首要依据。与玉米叶绿体的23S rDNA以及人类和小鼠线粒体的16S(大亚基)rDNA进行序列比较,使得实验模型得以改进并外推,从而得出所有这四个物种的完整二级结构。这些结构由明确界定的结构域组成,两个23S物种之间有超过450个补偿性碱基变化。还发现了一些核糖体结构“开关”,其中一个涉及5S rRNA。