Ekerot L, Ohlsson K
Scand J Plast Reconstr Surg. 1982;16(2):117-22. doi: 10.3109/02844318209006579.
A specific radioimmunoassay (RIA) was utilized to determine granulocyte elastase in the synovial fluid of a series of 45 rheumatoid joints in 39 patients. The average concentration was approximately 340 micrograms/l, exceeding that of the paired serum sample in all but 6 cases. No correlation was noted in synovial fluid between its content of granulocyte elastase and the total number of white blood cells. The concentrations in sera from rheumatoid patients with non-steroidal anti-inflammatory agents and controls were equivalent. Non-inflammatory exudates contained low concentrations of elastase. The immunoreactive granulocyte elastase in rheumatoid synovial fluid was bound to alpha 1-antitrypsin suggesting release of the enzyme in its active form. An uptake of elastase-containing complexes by macrophage-like cells in the synovial membrane was demonstrated by immunohistochemical staining of tissue using antiserum against granulocyte elastase. The concentrations of immunoreactive granulocyte elastase in the free synovial fluid of rheumatoid arthritic joints presumably only faintly reflect the situation concerning the target substrates.
采用一种特定的放射免疫分析法（RIA）测定了39例患者45个类风湿关节滑液中的粒细胞弹性蛋白酶。平均浓度约为340微克/升，除6例之外，均超过配对血清样本的浓度。滑液中粒细胞弹性蛋白酶含量与白细胞总数之间未发现相关性。类风湿患者使用非甾体抗炎药后的血清浓度与对照组相当。非炎性渗出液中弹性蛋白酶浓度较低。类风湿滑液中的免疫反应性粒细胞弹性蛋白酶与α1-抗胰蛋白酶结合，提示该酶以活性形式释放。通过使用抗粒细胞弹性蛋白酶抗血清对组织进行免疫组化染色，证实滑膜中巨噬细胞样细胞摄取了含弹性蛋白酶的复合物。类风湿关节炎关节游离滑液中免疫反应性粒细胞弹性蛋白酶的浓度可能仅微弱反映了与靶底物有关的情况。
