Pestana De Castro A F, Gomes J A, Serafim M B, Queiroz Tavares D, Lemos Dos Reis M H
Ann Immunol (Paris). 1982 Jul-Aug;133D(1):103-17.
An inflammatory skin reaction similar to the permeability factor (PF) described for the thermolabile (TL) enterotoxin of Escherichia coli was induced in rabbits inoculated intradermally with supernatants from cultures of Salmonella typhimurium and S. enteritidis. This PF-like activity was observed with both crude supernatants as well as those which were submitted to gel filtration through Sephadex G-100. PF-like activity was found only in fraction 1 (F1) of the chromatographed materials. It was resistant to boiling, proteolytic enzymes and wide variations of pH. Serological studies based on agglutination and immunodiffusion tests demonstrated that F1 materials were closely related to the somatic antigen of group B Salmonella. No specific TL activity, as detected by the Y-1 adrenal cell assay and the passive immune haemolysis test, could be demonstrated. Furthermore, F1 materials were not enterotoxigenic as assayed by the rabbit ileal loop assay, and no neutralization of PF-like activity could be obtained in tests carried out using F1 preparations pre-incubated with either anti-F1 or cholera antitoxin. Based upon these findings, it seems reasonable to suppose that most PF reactions, already reported as being caused by a TL-like enterotoxin produced by Salmonella, are probably due to endotoxin. In fact, this possibility was reinforced by the Sanarelli-Shwartzman phenomenon which was produced in rabbits inoculated with F1 materials.
给家兔皮内接种鼠伤寒沙门氏菌和肠炎沙门氏菌培养物的上清液后，诱导出了一种类似于大肠杆菌不耐热（TL）肠毒素所描述的渗透因子（PF）的炎性皮肤反应。无论是粗制上清液还是经葡聚糖G - 100凝胶过滤的上清液，均观察到了这种PF样活性。PF样活性仅在层析材料的第1组分（F1）中被发现。它对煮沸、蛋白水解酶和广泛的pH变化具有抗性。基于凝集试验和免疫扩散试验的血清学研究表明，F1材料与B组沙门氏菌的菌体抗原密切相关。通过Y - 1肾上腺细胞试验和被动免疫溶血试验检测，未发现特异性TL活性。此外，通过兔回肠袢试验测定，F1材料不具有肠毒素活性，并且在使用预先与抗F1或霍乱抗毒素孵育的F1制剂进行的试验中，未获得PF样活性的中和作用。基于这些发现，推测大多数已报道为由沙门氏菌产生的TL样肠毒素引起的PF反应可能是由内毒素引起的，这似乎是合理的。事实上，在用F1材料接种的家兔中产生的沙纳雷利 - 施瓦茨曼现象进一步证实了这种可能性。
