Kirchman D, Ducklow H, Mitchell R
Appl Environ Microbiol. 1982 Dec;44(6):1296-307. doi: 10.1128/aem.44.6.1296-1307.1982.
Rates of nucleic acid synthesis have been used to examine microbiol growth in natural waters. These rates are calculated from the incorporation of [3H]adenine and [3H]thymidine for RNA and DNA syntheses, respectively. Several additional biochemical parameters must be measured or taken from the literature to estimate growth rates from the incorporation of the tritiated compounds. We propose a simple method of estimating a conversion factor which obviates measuring these biochemical parameters. The change in bacterial abundance and incorporation rates of [3H]thymidine was measured in samples from three environments. The incorporation of exogenous [3H]thymidine was closely coupled with growth and cell division as estimated from the increase in bacterial biomass. Analysis of the changes in incorporation rates and initial bacterial abundance yielded a conversion factor for calculating bacterial production rates from incorporation rates. Furthermore, the growth rate of only those bacteria incorporating the compound can be estimated. The data analysis and experimental design can be used to estimate the proportion of nondividing cells and to examine changes in cell volumes.
核酸合成速率已被用于检测天然水体中的微生物生长情况。这些速率分别通过[3H]腺嘌呤和[3H]胸腺嘧啶核苷掺入RNA和DNA合成中的情况来计算。为了从氚化化合物的掺入情况估算生长速率，还必须测量或从文献中获取其他几个生化参数。我们提出了一种估算转换因子的简单方法，该方法无需测量这些生化参数。在来自三种环境的样本中，测量了细菌丰度的变化以及[3H]胸腺嘧啶核苷的掺入速率。从细菌生物量的增加估算，外源[3H]胸腺嘧啶核苷的掺入与生长和细胞分裂密切相关。对掺入速率和初始细菌丰度变化的分析得出了一个转换因子，用于从掺入速率计算细菌生产率。此外，仅能估算出那些掺入该化合物的细菌的生长速率。数据分析和实验设计可用于估算不分裂细胞的比例，并检测细胞体积的变化。
