Holán V, Hasek M, Chutná J, Holoubková I
Folia Biol (Praha). 1982;28(6):395-408.
Transplantation tolerance was induced in A.TL mice to donors having disparity in the Ia antigens and identity at the H-2K, H-2D and non-H-2 antigens. After neonatal injection of 12 X 10(6) semiallogeneic lymphoid cells, permanent survival of A.TH skin allografts was observed in more than 90% of the recipients. Adoptive transfer of 50 X 10(6) lymphoid cells from normal A.TL donors to tolerant mice resulted in rejection of the tolerated grafts only in half of the animals. When cells from tolerant mice were tested in MLC and GVH assays, they reacted positively as did cells from normal mice. After sensitization in vitro, cells from tolerant mice were cytotoxic to A.TH antigens. Serum from tolerant mice did not inhibit cell proliferation in MLC assay nor blocked cytotoxic reaction in vitro and also did not enhance survival of A.TH skin grafts in normal A.TL mice. When the enhancing effect of this serum was tested in the recipients treated with ALS, prolonged survival of allografts was observed. Attempts to prolong survival of A.TH skin allografts by transfer of spleen cells from tolerant donors failed in normal A.TL recipients, while they were successful in the recipients treated with ALS. Long-term tolerated A.TH allografts, when transferred to normal A.TL recipients, were rejected. The findings show that loss of antigenicity of the tolerated skin allografts is not the mechanism of tolerance in this model and that cells capable of recognizing antigens of the tolerated allografts and reacting against them are still present in tolerant animals. Tolerance of skin allografts disparate only in Ia antigens, as has been shown at least for the strain combination tested, is probably mediated by the positive serum and cell suppressor mechanisms that block in vivo the effector phase of allotransplantation reaction.
在A.TL小鼠中诱导出对Ia抗原存在差异而H-2K、H-2D和非H-2抗原相同的供体的移植耐受。新生小鼠注射12×10⁶个半同种异体淋巴细胞后，在超过90%的受体中观察到A.TH皮肤同种异体移植物的永久存活。将50×10⁶个来自正常A.TL供体的淋巴细胞过继转移到耐受小鼠中，仅在一半的动物中导致耐受移植物被排斥。当在混合淋巴细胞培养（MLC）和移植物抗宿主（GVH）试验中检测耐受小鼠的细胞时，它们的反应与正常小鼠的细胞一样呈阳性。在体外致敏后，耐受小鼠的细胞对A.TH抗原具有细胞毒性。耐受小鼠的血清在MLC试验中不抑制细胞增殖，在体外也不阻断细胞毒性反应，并且也不提高正常A.TL小鼠中A.TH皮肤移植物的存活率。当在接受抗淋巴细胞血清（ALS）治疗的受体中测试这种血清的增强作用时，观察到同种异体移植物的存活时间延长。通过转移来自耐受供体的脾细胞来延长A.TH皮肤同种异体移植物存活时间的尝试在正常A.TL受体中失败了，而在接受ALS治疗的受体中则成功了。长期耐受的A.TH同种异体移植物转移到正常A.TL受体中时会被排斥。这些发现表明，在该模型中，耐受皮肤同种异体移植物抗原性的丧失不是耐受的机制，并且能够识别耐受同种异体移植物抗原并对其作出反应的细胞仍然存在于耐受动物中。至少在所测试的品系组合中，仅Ia抗原存在差异的皮肤同种异体移植物的耐受可能是由阳性血清和细胞抑制机制介导的，这些机制在体内阻断同种异体移植反应的效应阶段。
