Hasek M, Holán V, Chutná J
Folia Biol (Praha). 1980;26(3):167-75.
Transplantation tolerance induced by the semiallogeneic cells in newborn mice or by the adoptive transfer of syngeneic spleen cells from neonatally tolerant donors in adult mice was studied in the strain combination with the H-2D region disparity (B10.A recipients--B10.A(2R) donors). Tolerance could be transfered adoptively already from 3-week-old mice that had been rendered tolerant at birth and the ability for the transfer of tolerance persisted for long periods even when neonatally tolerant animals were not skin grafted. Both neonatally and adoptively induced tolerance could not be abolished by the adoptive transfer of 100 x 10(6) immunocompetent cells from normal syngeneic donors. It was observed in the in vitro experiments that cells from tolerant mice in the two types of tolerance reacted to the tolerated antigens in the mixed lymphocyte culture, did not react to the tolerated antigens in the microcytotoxicity test (only some mice with adoptively induced tolerance showed a certain degree of reactivity), but cells from both types of tolerant mice inhibited the in vitro sensitization of cells from normal syngeneic animals. This suppression was stronger with cells from neonatally tolerant mice.
在具有H-2D区域差异的品系组合（B10.A受体——B10.A(2R)供体）中，研究了新生小鼠中的半同种异体细胞或成年小鼠中来自新生期耐受供体的同基因脾细胞的过继转移所诱导的移植耐受。耐受可以从出生时即已耐受的3周龄小鼠过继转移，并且即使新生期耐受的动物未进行皮肤移植，耐受转移能力也能长期持续。新生期和过继诱导的耐受都不能通过来自正常同基因供体的100×10(6)个免疫活性细胞的过继转移而被消除。在体外实验中观察到，两种耐受类型中耐受小鼠的细胞在混合淋巴细胞培养中对耐受抗原产生反应，在微量细胞毒性试验中对耐受抗原不产生反应（只有一些过继诱导耐受的小鼠表现出一定程度的反应性），但两种耐受类型小鼠的细胞都抑制了正常同基因动物细胞的体外致敏。新生期耐受小鼠的细胞这种抑制作用更强。
