重离子对酵母细胞的影响：刀豆氨酸抗性突变体的诱导

Heavy ion effects on yeast cells: induction of canavanine-resistant mutants.

作者信息

Kiefer J, Rase S, Schneider E, Straaten H, Kraft G, Liesem H

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Dec;42(6):591-600. doi: 10.1080/09553008214551571.

Abstract

The induction of forward mutations (resistance to canavanine) by heavy ion bombardment was investigated in wild type haploid yeast Saccharomyces cerevisiae. Accelerated ions of argon, titanium, nickel, krypton, xenon, lead and uranium with specific energies between 1.7 and 9.25 MeV/u were obtained from the UNILAC machine at the Gesellschaft für Schwerionenforschung, Darmstadt/Germany. LET-values ranged from 1200 to about 15 000 keV/microns. There was no unequivocal dependence of mutation induction cross section on either LET or Z*2/beta 2, but also a prominent influence of ion specific energy. This is explained by the action of long-ranging delta-electrons.

摘要

在野生型单倍体酿酒酵母中研究了重离子轰击诱导正向突变（对刀豆氨酸的抗性）的情况。从德国达姆施塔特重离子研究中心的UNILAC加速器获得了能量在1.7至9.25 MeV/u之间的氩、钛、镍、氪、氙、铅和铀的加速离子。传能线密度（LET）值范围为1200至约15000 keV/微米。突变诱导截面与LET或Z*2/beta 2均无明确的相关性，但离子比能有显著影响。这可以用长程δ电子的作用来解释。