Ikpeme S, Löbrich M, Akpa T, Schneider E, Kiefer J
Strahlenzentrum der Justus-Liebig-Universität Giessen, Germany.
Radiat Environ Biophys. 1995 Jun;34(2):95-9. doi: 10.1007/BF01275213.
Cells of diploid yeast, Saccharomyces cerevisiae, were exposed to a variety of energetic heavy ions (provided by the UNILAC facility at the Gesellschaft für Schwerionenforschung, GSI), 241Am alpha-particles and 80-keV x-rays after which they were assessed for DNA double-strand breaks (DSB) using either the neutral sedimentation or the pulsed-field gel electrophoresis (PFGE) technique. Both yielded comparable results. The DSB production cross-sections are compared with inactivation studies performed for the same cells under identical conditions. The measurements show that with lighter ions DSB induction cross-sections increase with linear energy transfer (LET), but the situation is less clear with the heavier ions. A close parallelism was found between DSB induction and cell inactivation in these yeast cells.
将二倍体酵母酿酒酵母细胞暴露于多种高能重离子（由德国重离子研究中心的UNILAC装置提供）、²⁴¹Amα粒子和80 keV X射线，之后使用中性沉降或脉冲场凝胶电泳（PFGE）技术评估它们的DNA双链断裂（DSB）情况。两种方法得到了类似的结果。将DSB产生截面与在相同条件下对相同细胞进行的失活研究进行比较。测量结果表明，对于较轻的离子，DSB诱导截面随传能线密度（LET）增加而增大，但对于较重的离子，情况不太明确。在这些酵母细胞中发现DSB诱导与细胞失活之间存在密切的平行关系。
