Thiéry G, Bergeron M
Rev Can Biol. 1976 Dec;35(4):211-6.
A new histological technique of block staining based on the use of a double lead and copper citrate is described in order to observe thick sections (0.5-1.5 micron) with a transmission electron microscope. With this technique, a network of mitochondrial with many morphological communications was seen in the epithelial cells of the proximal and distal convoluted tubules of the rat nephron. A new schematic view of the proximal and distal tubular cell is presented to illustrate the communications of the chondriome, which could become more or less accentuated following the functional status of the cell.
本文描述了一种基于使用双铅和柠檬酸铜的新型组织块染色技术,以便用透射电子显微镜观察厚切片(0.5 - 1.5微米)。通过该技术,在大鼠肾单位近端和远端曲管的上皮细胞中可见到具有许多形态学连通的线粒体网络。本文还呈现了近端和远端肾小管细胞的新示意图,以说明线粒体的连通情况,其可能会根据细胞的功能状态或多或少地增强。