Moretti R, Vujovic A, Benda N
Quad Sclavo Diagn. 1982 Jun;18(2):209-14.
Two tests of the 3rd generation were evaluated for the identification of HBsAg: the reverse hemoagglutination and the enzymatic immunoassay. The results of the study, carried out on 2,434 sera of occasional and periodic donors, have been slightly discordant. It was confirmed the greater sensibility of the IEA test over the RHA and there was a high incidence of false positive results of the IEA test probably caused by the presence in the sera of an elevated concentration of lipid and/or Hb. However, we can consider the RHA a useful test adaptable as an emergency test while the IEA method, being more sensible, can be used as a basic screening test.
对第三代用于鉴定乙肝表面抗原(HBsAg)的两种检测方法进行了评估:反向血凝试验和酶免疫测定法。该研究对2434份临时和定期献血者的血清进行检测,结果略有不一致。证实了酶免疫测定法比反向血凝试验具有更高的灵敏度,并且酶免疫测定法假阳性结果的发生率较高,这可能是由于血清中脂质和/或血红蛋白浓度升高所致。然而,我们可以认为反向血凝试验是一种有用的检测方法,适用于应急检测,而酶免疫测定法由于更灵敏,可作为基本的筛查检测方法。