Mancal P, Benda R, König J, Helm R, Nĕmecek V, Kyselová M
J Hyg Epidemiol Microbiol Immunol. 1983;27(4):425-35.
Two variants of sandwich-type ELISA (Enzyme Linked Immunosorbent Assay) kits for HBsAg detection (Sevatest ELISA HBsAg Macro I and Sevatest ELISA HBsAg Micro I) in human sera and plasmas were developed. As the solid phase, the ELISA Macro kit and ELISA Micro kit make use of polystyrene microtubes, and polystyrene microtitration plates, respectively, of Czechoslovak production (Koh-i-noor, Dalecín). Capture anti HBs antibody for adsorption to solid phase and rabbit anti HBs antibody for labelling with horse-radish peroxidase were prepared for both tests. The sensitivity of both ELISA kits for HBsAg, equal to approx. 2 ng/ml, was determined by titrating six selected HBsAg-positive sera and the WHO Agk 76 panel of HBsAg-positive sera and the results were compared with those obtained by ELISA, RIA (Radioimmunoassay) and RPHA (Reverse passive hemagglutination) kits of different producers and by counter-immunoelectrophoresis (CIEP). The sensitivity of the new ELISA kits was comparable to that of other producers' ELISA kits, higher than that of RPHA kits and only a little lower than that of RIA kits. A set of sera of patients hospitalised with different diagnoses was tested for HBsAg. The detection rate by ELISA Macro kit 2.8 and 1.5 times higher than by CIEP and RPHA (Raphadex B), respectively, and 1.1 time lower than by RIA (Austria II).
开发了两种用于检测人血清和血浆中乙肝表面抗原（HBsAg）的夹心型酶联免疫吸附测定（ELISA）试剂盒变体（Sevatest ELISA HBsAg Macro I和Sevatest ELISA HBsAg Micro I）。作为固相，ELISA Macro试剂盒和ELISA Micro试剂盒分别使用捷克斯洛伐克生产的（Koh-i-noor，Dalecín）聚苯乙烯微量管和聚苯乙烯微量滴定板。两种检测均制备了用于吸附到固相的捕获抗HBs抗体和用于用辣根过氧化物酶标记的兔抗HBs抗体。通过滴定六种选定的HBsAg阳性血清和世界卫生组织HBsAg阳性血清Agk 76组来确定两种ELISA试剂盒对HBsAg的敏感性，约为2 ng/ml，并将结果与不同生产商的ELISA、放射免疫测定（RIA）和反向被动血凝试验（RPHA）试剂盒以及对流免疫电泳（CIEP）所获得的结果进行比较。新ELISA试剂盒的敏感性与其他生产商的ELISA试剂盒相当，高于RPHA试剂盒，仅略低于RIA试剂盒。对一组因不同诊断住院的患者血清进行了HBsAg检测。ELISA Macro试剂盒的检测率分别比CIEP和RPHA（Raphadex B）高2.8倍和1.5倍，比RIA（奥地利II）低1.1倍。
