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[大鼠微循环中毛细血管内白细胞聚集的测量:细胞提取及单核细胞富集特性]

[Measurement of leukocyte accumulation in the capillaries in the lesser circulation of the rat: extraction of cells and characterization of enrichment in monocytes].

作者信息

Fritsch P, Masse R

出版信息

Biomed Pharmacother. 1982;36(5):259-63.

PMID:6762888
Abstract

We have performed a qualitative and quantitative study of leucocytes in lung alveolar capillaries. This population is not an artefact induced by experimental procedure because the same population is observed after instant cardiac arrest. About 30% of these leucocytes can be extracted by perfusion, the extracted population being representative of the whole one present in capillaries. The experimental procedure used allow to collect 100.10(6) leucocytes per animal 15.10(6) of which are monocytes easily obtained as a purified population. From these results lung capillaries contain at least as many leucocytes as the whole circulating blood and 5 times more monocytes.

摘要

我们对肺泡毛细血管中的白细胞进行了定性和定量研究。该细胞群体并非实验操作所致的假象,因为在心脏骤停瞬间后可观察到相同的细胞群体。这些白细胞中约30%可通过灌注提取,所提取的细胞群体代表了毛细血管中存在的整个细胞群体。所采用的实验方法能够使每只动物收集到100×10⁶个白细胞,其中15×10⁶个是单核细胞,很容易获得纯化的单核细胞群体。从这些结果来看,肺毛细血管中的白细胞数量至少与整个循环血液中的一样多,且单核细胞数量是其5倍。

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