Ehlers N, Sperling S, Olsen T
Acta Ophthalmol (Copenh). 1982 Dec;60(6):935-44. doi: 10.1111/j.1755-3768.1982.tb00624.x.
Seventeen corneas obtained 2-72 h post mortem, from donors aged 17-78 years, were cultivated for 24 h, cryopreserved, thawed, cultivated for another 24 h and grafted. One year post-operatively 12 of the 17 grafts were clear. In 10 of these 12 cases the visual acuity was greater than or equal to 0.33. One primary graft failure occurred, while 4 primarily clear grafts became cloudy due to glaucoma (2), phthisis bulbi (1) and herpetic reinfection (1). One year after the transplantations the central thickness of the clear grafts was 0.51 mm, and the endothelial cell density was 1028 cells/mm2, corresponding to 32% of the cell density before cryopreservation. This endothelial cell loss was not correlated to donor age or to the time between the death of the donor and the primary cultivation.
从17至78岁供体获取的17只角膜在死后2至72小时内采集，培养24小时，冷冻保存，解冻，再培养24小时后进行移植。术后一年，17只移植角膜中有12只保持透明。在这12例中，10例视力大于或等于0.33。发生了1例原发性移植失败，而4只原发性透明的移植角膜因青光眼（2例）、眼球痨（1例）和疱疹再感染（1例）而变得混浊。移植一年后，透明移植角膜的中央厚度为0.51毫米，内皮细胞密度为1028个细胞/平方毫米，相当于冷冻保存前细胞密度的32%。这种内皮细胞损失与供体年龄或供体死亡与初次培养之间的时间无关。
