一种用于检测嗜异性抗体的酶联免疫吸附测定（ELISA）。
An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
作者信息
Hsu J F, Evans A S, Niederman J C, Cenabre L C
出版信息
Yale J Biol Med. 1982 Sep-Dec;55(5-6):429-36.
An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity.
已开发出一种酶联免疫吸附测定（ELISA）试验用于检测嗜异性抗体。微量滴定试验利用牛红细胞单层作为抗原，以及与辣根过氧化物酶结合的抗人IgM抗血清来测定试验血清中嗜异性抗体与红细胞的结合程度。当在分光光度计中读取时，单一血清稀释度可产生定量结果。ELISA试验显示出与免疫粘附血凝试验（IAHA）和其他嗜异性试验相当的敏感性、良好的重复性和高特异性。
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