Miceli M D, Schifaudo G, Vitale G, Di Rosa S, Mansueto S
Quad Sclavo Diagn. 1982 Sep;18(3):345-53.
The diagnosis of acute toxoplasmosis usually depends on serology, since clinical and/or histological features are difficult and/or often misleading. IgM titers are the best indicators of infection acquired in the past two to four months. IgG titers are generally correlated with non-active infections as well as previous (symptomatic and/or asymptomatic) illness. In the present paper, results obtained with a new kit for the evaluation of IgG and specific-IgM by micro-ELISA technique, are reported. Optical density values in sera from: a) blood donors; b) miscellany group; c) suspected toxoplasmosis, were at various degree high for IgG. Few sera of the entire sample (in the group of suspected toxoplasmosis and one in miscellany) showed high optical density for specific IgM. Reading of results with spectrophotometer were in agreement with reading to the naked eye. Reproducibility was satisfactory. Unfortunately some sera positive in the first determinations gave equivocal results in successive proofs. For these reasons diagnosis of toxoplasmosis must be based upon the use of at least three tests.
急性弓形虫病的诊断通常依赖于血清学检查，因为临床和/或组织学特征难以判断且常常具有误导性。IgM滴度是过去两到四个月内感染的最佳指标。IgG滴度通常与非活动性感染以及既往（有症状和/或无症状）疾病相关。本文报道了使用一种新型试剂盒通过微量ELISA技术评估IgG和特异性IgM所获得的结果。来自以下人群血清的光密度值：a）献血者；b）混合组；c）疑似弓形虫病患者，其IgG光密度值在不同程度上偏高。在整个样本中（疑似弓形虫病组和混合组中的一份样本），只有少数血清的特异性IgM光密度值较高。用分光光度计读取结果与肉眼读取结果一致。重复性良好。不幸的是，一些首次检测呈阳性的血清在后续检测中结果不明确。因此，弓形虫病的诊断必须至少基于三项检测。
