Marschalkó M, Barna-Vetró I, Horváth I
Acta Microbiol Acad Sci Hung. 1982;29(4):221-6.
Enzyme-linked immunosorbent assay using ultrasonic lysate of Treponema pallidum and Treponema reiteri as antigens was used for the detection of antisyphilitic antibodies in various stages of syphilis. The conjugate was goat antiserum to human IgG labelled with horseradish peroxidase. A comparison with results of the T. pallidum immobilization test, Rapid Plasma Reagin test, Kolmer complement fixation reaction using cardiolipin and Reiter protein antigens showed that ELISA was more sensitive but less specific with T. pallidum antigen, whereas less sensitive but more specific with T. reiteri antigen. Absorption of group specific treponemal antibodies was needed to make the method reliable.
以梅毒螺旋体和里氏螺旋体的超声裂解物为抗原的酶联免疫吸附测定法，用于检测梅毒各阶段的抗梅毒抗体。结合物是用辣根过氧化物酶标记的山羊抗人IgG抗血清。与梅毒螺旋体制动试验、快速血浆反应素试验、使用心磷脂和里氏蛋白抗原的柯尔默补体结合反应结果进行比较表明，酶联免疫吸附测定法用梅毒螺旋体抗原时更敏感但特异性较低，而用里氏螺旋体抗原时敏感性较低但特异性较高。需要吸收类属特异性梅毒螺旋体抗体以使该方法可靠。
