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梅毒诊断中的电免疫扩散反应(作者译)

[Electroimmunodiffusion reaction in the diagnosis of syphilis (author's transl)].

作者信息

Tringali G, Vetro N, Intonazzo V

出版信息

Quad Sclavo Diagn. 1977 Dec;13(4):496-503.

PMID:351696
Abstract

A group of 800 human sera, obtained from syphilitic and nonsyphilitic individuals were tested in the counterimmunoelectrophoresis (CIE) technique with Reiter protein antigen. The sera were assayed for comparison in the following treponemal and nontreponemal tests: standard serologic tests for syphilis with cardiolipin; complement fixation (Kolmer 1/5 vol) with Reiter protein antigen (RPCF); T. pallidium immobilization (TPI) test; haemoagglutination test for T. pallidum antibodies (TPHA). The sensitivity and specificity of CIE test, its simplicity, the low cost, the possibility of practical application as a routine test for syphilis serology were discussed.

摘要

从梅毒患者和非梅毒患者中获取了一组800份人血清样本,采用对流免疫电泳(CIE)技术,以赖特蛋白抗原进行检测。对这些血清样本进行了以下梅毒螺旋体和非梅毒螺旋体检测以作比较:用磷脂酰胆碱进行梅毒标准血清学检测;用赖特蛋白抗原进行补体结合试验(柯尔默1/5体积法)(RPCF);梅毒螺旋体制动试验(TPI);梅毒螺旋体抗体血凝试验(TPHA)。讨论了CIE检测的敏感性和特异性、其简易性、低成本以及作为梅毒血清学常规检测实际应用的可能性。

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