Kubasova T, Somosy Z, Bertók L, Köteles G J
Acta Microbiol Acad Sci Hung. 1982;29(4):235-43.
The effect of parent lipopolysaccharide (LPS) and radio-detoxified endotoxin (rdLPS) on various human blood cell membranes proved to be different as detected by 3H-concanavalin A-binding technique in vitro. The lectin-binding ability of erythrocyte membranes did not change upon treatment by either endotoxin, whereas that of lymphocytes was stimulated by LPS at 10-50 micrograms/ml concentration as well as by rdLPS at the lowest dose applied, i.e. 0.1 micrograms/ml. The LPS-treated platelets bound 3H-concanavalin A less than did the untreated controls; on the other hand, the rdLPS did not change the lectin-binding surface of these cells. The affection by radiation of cell membranes could be prevented by pretreatment with endotoxins. This fact, however, could not be considered a radioprotective effect. The micromorphological investigations by scanning electron microscopy (SEM) support our data concerning the functional alterations of plasma membranes of platelets and lymphocytes after LPS and rdLPS treatment as well as after the combined effect of endotoxin pretreatment and X-irradiation, since a severe smoothening of the cell surface could be observed.
通过体外3H-伴刀豆球蛋白A结合技术检测发现，亲本脂多糖（LPS）和辐射解毒内毒素（rdLPS）对各种人体血细胞的细胞膜的影响有所不同。红细胞膜经任何一种内毒素处理后，其凝集素结合能力均未改变，而淋巴细胞的凝集素结合能力在浓度为10 - 50微克/毫升的LPS以及最低应用剂量即0.1微克/毫升的rdLPS作用下受到刺激。经LPS处理的血小板结合3H-伴刀豆球蛋白A的能力比未处理的对照血小板弱；另一方面，rdLPS并未改变这些细胞的凝集素结合表面。内毒素预处理可防止细胞膜受到辐射的影响。然而，这一事实不能被视为辐射防护作用。扫描电子显微镜（SEM）的微观形态学研究支持了我们关于LPS和rdLPS处理后以及内毒素预处理和X射线照射联合作用后血小板和淋巴细胞质膜功能改变的数据，因为可以观察到细胞表面严重平滑化。
