转运RNA的结构变异性：酵母苯丙氨酸转运RNA-大肠杆菌谷氨酸转运RNA2复合物的小角X射线散射
Structural variability of tRNA: small-angle x-ray scattering of the yeast tRNAphe-Escherichia coli tRNAGlu2 complex.
作者信息
Nilsson L, Rigler R, Laggner P
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5891-5. doi: 10.1073/pnas.79.19.5891.
Abstract
The structure of the complex formed in solution between yeast tRNAPhe and Escherichia coli tRNAGlu2 has been studied by small-angle x-ray scattering. The complex has a radius of gyration of 4.0 nm and an electron-pair distance distribution that is incompatible with a model composed to two tRNAs joined at their complementary anticodons and exhibiting the L shape seen in the crystal. Instead a model in which the two tRNAs, still bound via the anticodons, assume a conformation with the acceptor arms folded toward the anticodon arms agrees with the observed scattering curves.
摘要
通过小角X射线散射研究了酵母苯丙氨酸转运核糖核酸（tRNAPhe）与大肠杆菌谷氨酸转运核糖核酸2（tRNAGlu2）在溶液中形成的复合物的结构。该复合物的回转半径为4.0纳米，其电子对距离分布与由两个在互补反密码子处连接并呈现晶体中所见L形的转运核糖核酸组成的模型不相符。相反，一种模型认为，这两个仍通过反密码子结合的转运核糖核酸呈现出一种构象，其中受体臂朝着反密码子臂折叠，这与观察到的散射曲线相符。
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