Jonak Z L, Kennett R H, Bechtol K B
Hybridoma. 1982;1(4):349-68. doi: 10.1089/hyb.1.1982.1.349.
Two different types of monoclonal antibodies, antineuroblastoma (PI153/3), and antilymphocyte (P3B1-C3) were used to identify and classify tumor cells in the bone marrow of patients with neuroblastoma and with other types of cancer. Cells expressing the antigens were detected with peroxidase-coupled anti-Ig. The cell-surface labeling is manifested as a dense black precipitate at the membrane visualized by light microscopy. The combination of the two antibodies gives specific staining patterns for each cell type. PI153/3+, P3B1-C3- is specifically associated with neuroblastoma cells. PI153/3+,P3B1-C3+ is expressed on blast cells from some types of acute lymphoblastic leukemia and a small subpopulation of normal lymphocytes. These monoclonal antibodies thus allow specific visual detection of single neuroblastoma cells in bone marrow samples. The results demonstrate how combinations of monoclonal antibodies can be effectively used to identify specific cell types by their expression of and lack of specific marker determinants. Application of this principle is particularly relevant for dissecting populations of related cells and/or molecules.
使用两种不同类型的单克隆抗体，即抗神经母细胞瘤（PI153/3）和抗淋巴细胞（P3B1 - C3），对神经母细胞瘤患者及其他类型癌症患者骨髓中的肿瘤细胞进行鉴定和分类。用与过氧化物酶偶联的抗Ig来检测表达抗原的细胞。细胞表面标记表现为在光镜下可见的细胞膜处密集的黑色沉淀。两种抗体的组合为每种细胞类型提供了特定的染色模式。PI153/3 +、P3B1 - C3 - 与神经母细胞瘤细胞特异性相关。PI153/3 +、P3B1 - C3 + 在某些类型的急性淋巴细胞白血病的原始细胞以及一小部分正常淋巴细胞上表达。因此，这些单克隆抗体能够在骨髓样本中特异性地可视化检测单个神经母细胞瘤细胞。结果表明，单克隆抗体的组合如何能够通过细胞对特定标志物决定簇的表达和不表达有效地用于鉴定特定细胞类型。这一原理的应用对于剖析相关细胞和/或分子群体尤为重要。
