用单克隆抗体鉴定和分离黑色素瘤相关抗原。
Identification and isolation of melanoma-associated antigens with monoclonal antibodies.
作者信息
Ross A H, Mitchell K F, Steplewski Z, Koprowski H
出版信息
Hybridoma. 1982;1(4):413-21. doi: 10.1089/hyb.1.1982.1.413.
The monoclonal antibodies distributed at the first Workshop on Monoclonal Antibodies to Melanoma have been tested by immunoprecipitation, and one group of antigens (90 kd) has been found to be identical by sequential immunoprecipitation. These results are in good agreement with those from other laboratories. In addition, antigens from a new group of hybridomas, generated since the first Workshop, were studied. Some of these antigens are unique and others are duplicates of the original set. Finally, a melanoma-associated antigen (260 kd) was purified by immunoaffinity chromatography.
在第一届黑色素瘤单克隆抗体研讨会上分发的单克隆抗体已通过免疫沉淀法进行检测，并且通过连续免疫沉淀法发现一组抗原（90kd）是相同的。这些结果与其他实验室的结果高度一致。此外，还研究了自第一届研讨会以来产生的一组新杂交瘤的抗原。其中一些抗原是独特的，而其他一些则是原始组的重复。最后，通过免疫亲和层析法纯化了一种黑色素瘤相关抗原（260kd）。
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