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Relaxed sequence specificities of Eco RI endonuclease and methylase: mechanisms, possible practical applications, and uses in defining protein-nucleic acid recognition mechanisms.

作者信息

Woodbury C P, von Hippel P H

机构信息

Institute of Molecular Biology, University of Oregon, Eugene 97403.

出版信息

Gene Amplif Anal. 1981;1:181-207.

PMID:6765639
Abstract
摘要

相似文献

1
Relaxed sequence specificities of Eco RI endonuclease and methylase: mechanisms, possible practical applications, and uses in defining protein-nucleic acid recognition mechanisms.
Gene Amplif Anal. 1981;1:181-207.
2
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[Interactions between Eco RI restriction endonuclease and DNA].[大肠杆菌限制性内切酶Eco RI与DNA之间的相互作用]
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Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA.
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Recognition by restriction endodeoxyribonuclease EcoRI of octadeoxyribonucleotides containing modified sugar and base moieties.限制性内切脱氧核糖核酸酶EcoRI对含有修饰糖基和碱基部分的十八脱氧核糖核苷酸的识别。
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The energetic basis of specificity in the Eco RI endonuclease--DNA interaction.Eco RI核酸内切酶与DNA相互作用中特异性的能量基础。
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Structures and mechanisms of Eco RI DNA restriction and modification enzymes.
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Molecular recognition mediated by bound water. A mechanism for star activity of the restriction endonuclease EcoRI.由结合水介导的分子识别。限制性内切酶EcoRI星号活性的一种机制。
J Mol Biol. 1993 Nov 20;234(2):302-6. doi: 10.1006/jmbi.1993.1586.
9
Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct.EcoRI对DNA的差异性识别与切割取决于丙二醛-脱氧鸟苷加合物两种形式之间的动态平衡。
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Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.对EcoRI限制性内切核酸酶中Gln115功能的突变分析,Gln115是识别序列-GAATTC-中内部胸腺嘧啶残基以及将特异性DNA结合与催化偶联的关键氨基酸。
J Mol Biol. 1993 Jan 5;229(1):221-34. doi: 10.1006/jmbi.1993.1019.

引用本文的文献

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The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases.保真度指数对DNA限制性核酸内切酶的星号活性进行了系统定量。
Nucleic Acids Res. 2008 May;36(9):e50. doi: 10.1093/nar/gkn182. Epub 2008 Apr 15.
2
Sequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II.DNA(胞嘧啶N4)甲基转移酶M.Pvu II基因的序列、内部同源性及高水平表达
Nucleic Acids Res. 1989 Jun 12;17(11):4161-75. doi: 10.1093/nar/17.11.4161.