Johanson W G, Higuchi J H, Chaudhuri T R, Woods D E
Am Rev Respir Dis. 1980 Jan;121(1):55-63. doi: 10.1164/arrd.1980.121.1.55.
Bacterial colonization of mucosal surfaces may be mediated by bacterial adherence to epithelial cells. To study the role of adherence in gram-negative bacillary colonization of the upper respiratory tract, we studied 32 noncolonized patients undergoing elective surgery. Adherence of Pseudomonas aeruginosa and 3 other bacilli to patients' buccal cells in vitro was studied pre- and postoperatively; results were correlated with occurrence of bacillary colonization of the oropharynx in vivo. Adherence of all species was similar. Preoperatively, mean +/- SD adherence was 4.3 +/- 2.0 Pseudomonas aeruginosa/cell. Postoperatively, adherence of Pseudomonas aeruginosa exceeded 8.3 (preoperative mean + 2 SD) bacilli/cell in 16 patients, 11 (69%) of whom became colonized. None of 16 patients whose cells adhered fewer than 8.3 bacilli/cell postoperatively became colonized. Buccal cell binding of 3H-concanavalin A was increased both pre- and postoperatively among patients who became colonized. Gram-negative bacillary colonization of the upper respiratory tract is associated with increased adherence of bacilli to buccal cells. Epithelial cell binding may provide the mechanism whereby ill patients are rendered susceptible to colonization.
细菌在黏膜表面的定植可能是由细菌黏附于上皮细胞介导的。为了研究黏附在上呼吸道革兰氏阴性杆菌定植中的作用，我们对32例接受择期手术的未定植患者进行了研究。术前和术后研究了铜绿假单胞菌和其他3种杆菌在体外对患者颊细胞的黏附情况；结果与体内口咽部杆菌定植的发生情况相关。所有物种的黏附情况相似。术前，铜绿假单胞菌的平均黏附量±标准差为4.3±2.0/细胞。术后，16例患者的铜绿假单胞菌黏附量超过8.3 （术前平均值+2个标准差）杆菌/细胞，其中11例（69%）发生了定植。术后颊细胞黏附杆菌少于8.3/细胞的16例患者均未发生定植。在发生定植的患者中，术前和术后3H-伴刀豆球蛋白A的颊细胞结合均增加。上呼吸道革兰氏阴性杆菌定植与杆菌对颊细胞黏附增加有关。上皮细胞结合可能提供了患病患者易发生定植的机制。
