Lee J C, King R D
Infect Immun. 1983 Sep;41(3):1024-30. doi: 10.1128/iai.41.3.1024-1030.1983.
Certain environmental, physical, and biochemical aspects of Candida albicans adherence to human vaginal epithelial cells were characterized by using an in vitro radiometric adherence assay. Blastospores harvested from cultures grown at 25 degrees C adhered to vaginal epithelial cells in significantly greater numbers than did blastospores isolated from cultures grown at 37 degrees C. C. albicans viability was not essential for adherence, but severe methods used to kill the blastospores did reduce their attachment. The addition of sodium chloride, divalent cations, sugars, mannan, or mannoprotein to the assay had no effect on attachment. Pretreatment of the blastospores with detergents, salts, urea, glycosidases, lipase, or pepsin did not affect adherence, but treatment with reducing agents or five proteolytic enzymes did render C. albicans nonadherent. Cell wall fragments prepared from C. albicans, but not from Candida krusei, adhered to vaginal epithelial cells. Loss of adherence after the cell walls were treated with alpha-mannosidase or papain suggests that cell wall mannoprotein is an essential component of the C. albicans adhesin.
通过体外放射性黏附试验，对白色念珠菌黏附人阴道上皮细胞的某些环境、物理和生化方面进行了表征。从25℃培养的培养物中收获的芽生孢子比从37℃培养的培养物中分离的芽生孢子黏附到阴道上皮细胞上的数量显著更多。白色念珠菌的活力对于黏附并非必不可少，但用于杀死芽生孢子的严苛方法确实会减少它们的附着。在试验中添加氯化钠、二价阳离子、糖、甘露聚糖或甘露糖蛋白对附着没有影响。用去污剂、盐、尿素、糖苷酶、脂肪酶或胃蛋白酶预处理芽生孢子不会影响黏附，但用还原剂或五种蛋白水解酶处理会使白色念珠菌不黏附。从白色念珠菌而非克鲁斯念珠菌制备的细胞壁片段黏附到阴道上皮细胞上。细胞壁用α-甘露糖苷酶或木瓜蛋白酶处理后黏附丧失，这表明细胞壁甘露糖蛋白是白色念珠菌黏附素的重要组成部分。
