有证据表明，由严紧反应诱导的枯草芽孢杆菌孢子形成是由GTP或GDP的减少引起的。
Evidence that Bacillus subtilis sporulation induced by the stringent response is caused by the decrease in GTP or GDP.
作者信息
Ochi K, Kandala J, Freese E
出版信息
J Bacteriol. 1982 Aug;151(2):1062-5. doi: 10.1128/jb.151.2.1062-1065.1982.
Abstract
Partial amino acid deprivation of Bacillus subtilis, which evokes the stringent response, initiates sporulation not because the highly phosphorylated guanine nucleotides guanosine-5'-diphosphate-3'-diphosphate (ppGpp) and guanosine-5'-triphosphate-3'-diphosphate (pppGpp) increase but because GTP decreases. This was shown with a mutant (Myc) partially resistant to mycophenolate, an inhibitor of IMP dehydrogenase. Upon amino acid deprivation, the Myc mutant (62032) showed the usual increase in ppGpp and pppGpp but a reduced decrease in GTP, and only few cells sporulated. Extensive sporulation was restored by the addition of mycophenolate or decoyinine, and inhibitor of GMP synthetase, which caused a further decrease in GTP.
摘要
枯草芽孢杆菌的部分氨基酸剥夺会引发严谨反应，其启动孢子形成并非因为高度磷酸化的鸟嘌呤核苷酸鸟苷 - 5'-二磷酸 - 3'-二磷酸（ppGpp）和鸟苷 - 5'-三磷酸 - 3'-二磷酸（pppGpp）增加，而是因为鸟苷三磷酸（GTP）减少。这一点在对霉酚酸（一种肌苷酸脱氢酶抑制剂）有部分抗性的突变体（Myc）中得到了证实。在氨基酸剥夺时，Myc突变体（62032）显示出ppGpp和pppGpp通常的增加，但GTP的减少幅度降低，并且只有少数细胞形成孢子。通过添加霉酚酸或6 - 重氮 - 5 - 氧代 - L - 正亮氨酸（一种鸟苷酸合成酶抑制剂）可恢复广泛的孢子形成，这会导致GTP进一步减少。
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