Strobel H W, Fang W F, Oshinsky R J
Cancer. 1980 Mar 15;45(5 Suppl):1060-5. doi: 10.1002/1097-0142(19800315)45:5+<1060::aid-cncr2820451305>3.0.co;2-e.
Rat colon mucosa microsomes contain a competent mixed function oxidase system that hydroxylates the N-methyl drugs benzphetamine and ethylmorphine, the O-alkyl drugs p-nitroanisole and p-nitrophenetole and the polycyclic carcinogen benzo[alpha]pyrene. The colon system's hydroxylation activities can be selectively induced by pretreatment with phenobarbital or beta-naphthoflavone and can be selectively inhibited by SKF-525A or 7,8-benzoflavone. The colon microsomal system has been solubilized with the non-ionic detergent Renex 690 and resolved by column chromatography into its components cytochrome P-450 and cytochrome P-450 reductase. Colon cytochrome P-450 and cytochrome P-450 reductase can be recombined to reconstitute hydroxylation activity. The colon system is also able to activate carcinogens to mutagenic metabolites as demonstrated in the Ames test system. In addition, the activity of the colon system is markedly increased by pretreatment with gastrointestinal hormones.
大鼠结肠黏膜微粒体含有一种活性混合功能氧化酶系统，该系统可使N-甲基药物苄非他明和乙基吗啡、O-烷基药物对硝基苯甲醚和对硝基苯乙醚以及多环致癌物苯并[a]芘发生羟基化反应。结肠系统的羟基化活性可通过苯巴比妥或β-萘黄酮预处理而被选择性诱导，也可被SKF-525A或7,8-苯并黄酮选择性抑制。结肠微粒体系统已用非离子去污剂Renex 690进行溶解，并通过柱色谱法分离成其组分细胞色素P-450和细胞色素P-450还原酶。结肠细胞色素P-450和细胞色素P-450还原酶可重新组合以重建羟基化活性。如在艾姆斯试验系统中所证实的，结肠系统也能够将致癌物激活为诱变代谢物。此外，通过胃肠道激素预处理可使结肠系统的活性显著增加。
