Saine S E, Fang W F, Strobel H W
Biochim Biophys Acta. 1978 Oct 12;526(2):345-58. doi: 10.1016/0005-2744(78)90126-2.
Novikoff kepatoma microsomes catalyze the hydroxylation of benzphetamine and ethylmorphine at rates less than 1% of those of liver microsomes but catalyze the hydroxylation of p-nitroanisole and p-nitrophenetole at rates about 40% of those of liver microsomes. Benzo[a]pyrene hydroxylation is also catalyzed by Novikoff hepatoma microsomes at about 2% of the rate of liver microsomes. Like the hepatic microsomal system the rates of substrate hydroxylation by Novikoff hepatoma microsomes can be increased by pretreatment with phenobarbital/hydrocortisone or beta-naphthoflavone and inhibited by carbon monoxide, SKF-525A, and 7,8-benzoflavone. In addition, NADPH-cytochrome P-450 reductase (NADPH:ferricytochrome oxidoreductase, EC 1.6.2.4) has been partially purified from Novikoff hepatoma ascites cells and some properties are described. The induction and inhibition characteristics of the Novikoff hepatoma microsomal hydroxylation activities and the isolation of a cytochrome P-450 reductase from the hepatoma are consistent with the presence of a functional mixed function oxidase system in the Novikoff hepatoma, analogous to that present in liver endoplasmic reticulum.
诺维科夫肝癌微粒体催化苄非他明和乙基吗啡的羟基化反应，其速率不到肝微粒体的1%，但催化对硝基苯甲醚和对硝基苯乙醚的羟基化反应，速率约为肝微粒体的40%。诺维科夫肝癌微粒体催化苯并[a]芘羟基化反应的速率约为肝微粒体的2%。与肝微粒体系统一样，诺维科夫肝癌微粒体对底物羟基化的速率可通过苯巴比妥/氢化可的松或β-萘黄酮预处理而增加，并受到一氧化碳、SKF-525A和7,8-苯并黄酮的抑制。此外，已从诺维科夫肝癌腹水细胞中部分纯化出NADPH-细胞色素P-450还原酶（NADPH：铁细胞色素氧化还原酶，EC 1.6.2.4），并描述了其一些特性。诺维科夫肝癌微粒体羟基化活性的诱导和抑制特性以及从肝癌中分离出细胞色素P-450还原酶，与诺维科夫肝癌中存在功能性混合功能氧化酶系统一致，类似于肝内质网中存在的系统。
