McDade R L, Johnston K H
J Bacteriol. 1980 Mar;141(3):1183-91. doi: 10.1128/jb.141.3.1183-1191.1980.
The proteins of the outer membrane of Neisseria gonorrhoeae play an important role in the serotyping system defined by K. H. Johnston et al. (J. Exp. Med. 143:741-758, 1976). This study attempted to delineate the molecular arrangement of the major proteins of the outer membrane of the gonococcus by using three approaches. First, natural protein-protein relationships were demonstrated by symmetrical, two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Second, proteins exposed on the surface of outer membrane vesicles were cross-linked by using the bifunctional reagents dimethyl-3,3'-dithiobispropionimidate and dithiobis[succinimidyl propionate]. Third, specific antigen-antibody interactions on the surface of membrane vesicles were analyzed by radioautographic techniques. The major proteins of the outer membrane of the gonococcus were defined, and a nomenclature was devised to take into account the effects of heat and reducing agents on the resolution of these proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results of cross-linking experiments strongly suggest that two of the major proteins of the gonococcal outer membrane (proteins 1 and 3) form a hydrophobically associated trimeric unit in situ which can be stabilized by selective cross-linking reagents. Results substantiated that these proteins are responsible for imparting serotypic specificity.
淋病奈瑟菌外膜蛋白在K. H. 约翰斯顿等人（《实验医学杂志》143:741 - 758, 1976）定义的血清分型系统中发挥重要作用。本研究试图通过三种方法来描绘淋球菌外膜主要蛋白的分子排列。首先，通过对称的二维十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳证明天然蛋白质 - 蛋白质关系。其次，使用双功能试剂二甲基 - 3,3'-二硫代双丙脒和二硫代双[琥珀酰亚胺丙酸酯]对外膜囊泡表面暴露的蛋白质进行交联。第三，通过放射自显影技术分析膜囊泡表面的特异性抗原 - 抗体相互作用。定义了淋球菌外膜的主要蛋白，并设计了一种命名法，以考虑加热和还原剂对这些蛋白在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中分辨率的影响。交联实验结果强烈表明，淋球菌外膜的两种主要蛋白（蛋白1和蛋白3）在原位形成疏水相关的三聚体单元，可通过选择性交联试剂使其稳定。结果证实这些蛋白赋予血清型特异性。
