Parsons N J, Kwaasi A A, Perera V Y, Patel P V, Martin P M, Smith H
J Gen Microbiol. 1982 Dec;128(12):3077-81. doi: 10.1099/00221287-128-12-3077.
The determinant(s) of gonococcal resistance to killing by human polymorphonuclear (PMN) phagocytes appear to be present in outer membrane vesicles (OMV) purified from lithium chloride extracts of Neisseria gonorrhoeae strain BS4 (agar) by wheat germ agglutinin (WGA) affinity chromatography. OMV neutralized the ability of antisera raised against whole gonococci to drastically reduce the capacity of strain BS4 (agar) to survive within PMN phagocytes. Furthermore, analysis by SDS-PAGE of OMV/WGA precipitates from lithium chloride extracts of strain BS4 (agar) and strain BSSH, which was more susceptible to phagocyte killing than strain BS4 (agar) and yielded OMV with poor antiserum-neutralizing activity, suggested that three proteins were associated with resistance to phagocyte killing.
淋病奈瑟菌菌株BS4（琼脂）经氯化锂提取后，通过麦胚凝集素（WGA）亲和层析法纯化得到的外膜囊泡（OMV）中似乎存在淋病奈瑟菌对人多形核（PMN）吞噬细胞杀伤作用产生抗性的决定因素。OMV中和了针对全菌淋病奈瑟菌产生的抗血清大幅降低菌株BS4（琼脂）在PMN吞噬细胞内存活能力的能力。此外，对菌株BS4（琼脂）和菌株BSSH（比菌株BS4（琼脂）更易被吞噬细胞杀伤且产生的OMV抗血清中和活性较差）的氯化锂提取物中的OMV/WGA沉淀物进行SDS-PAGE分析表明，三种蛋白质与对吞噬细胞杀伤的抗性有关。
