Khachapuridze G G, Mikhel'son V M, Zhestianikov V D
Tsitologiia. 1980 Mar;22(3):343-9.
A study was made of the effects of a chemical mutagen of the "gamma-type"--methylmethansulfonate (MMS) and of mutagen of the "UV-type"--4-nitroquinolin-1-oxide (NQO) and 7-brommethylbenz(alpha)antracen (BMBA) exerted on chromosome aberration frequency in lymphocytes of patients with classical Xeroderma pigmentosum and with a so-called form II of the disease on different stages of the cell cycle. Mutagens were added to PHA stimulated lymphocyte cultures every 3 hours, simultaneously with pulse 3H-thymidine labelling, to fix the stage of the cell cycle at the moment of treatment. NQO and BMBA treatments were found to increase the frequency of chromosome aberrations in classical XP cells, whereas MMS was not found to. In the XP II cells, defective in repair of both UV and gamma damaged DNA, chromosome aberrations yield is higher than in normal cells after all the three mutagens treatment. The data obtained show the correlation between DNA repair and chromosome aberrations yield.
对“γ型”化学诱变剂甲磺酸甲酯（MMS）以及“紫外线型”诱变剂4-硝基喹啉-1-氧化物（NQO）和7-溴甲基苯并（α）蒽（BMBA）对经典型着色性干皮病患者和该病所谓II型患者淋巴细胞染色体畸变频率在细胞周期不同阶段的影响进行了研究。诱变剂每3小时添加到PHA刺激的淋巴细胞培养物中，同时进行脉冲3H-胸腺嘧啶核苷标记，以确定处理时刻的细胞周期阶段。发现NQO和BMBA处理会增加经典型着色性干皮病细胞中的染色体畸变频率，而未发现MMS有此作用。在紫外线和γ射线损伤的DNA修复均有缺陷的着色性干皮病II型细胞中，经所有三种诱变剂处理后，染色体畸变率高于正常细胞。所得数据显示了DNA修复与染色体畸变率之间的相关性。
