离体灌注大鼠肝脏对[3H]硫酸软骨素4-硫酸酯的降解及[3H]己糖胺成分的再利用
Degradation of [3H]chondroitin 4-sulphate and re-utilization of the [3H]hexosamine component by the isolated perfused rat liver.
作者信息
MacNicholl A D, Wusteman F S, Winterburn P J, Powell G M, Curtis C G
出版信息
Biochem J. 1980 Jan 15;186(1):279-86. doi: 10.1042/bj1860279.
Abstract
Radiolabelled chondroitin 4-sulphate was isolated after incubation of rat rib cartilage with N-acetyl-D-[6-3H]galactosamine. After proteolytic digestion of the tissue with either papain or trypsin the released [3H]chondroitin 4-sulphate was added to an isolated perfused rat liver system. Analysis of perfusate after several hours perfusion showed that radiolabelled amino sugars were secreted by the liver in a low-molecular-weight form and as components of glycoproteins.
摘要
用 N-乙酰-D-[6-³H]半乳糖胺孵育大鼠肋软骨后，分离出放射性标记的硫酸软骨素 4。在用木瓜蛋白酶或胰蛋白酶对组织进行蛋白水解消化后，将释放的[³H]硫酸软骨素 4 添加到离体灌注大鼠肝脏系统中。灌注数小时后的灌注液分析表明，放射性标记的氨基糖以低分子量形式和糖蛋白成分的形式由肝脏分泌。
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