离体灌注大鼠肝脏对 N-乙酰-D-[1-¹⁴C]半乳糖胺和 N-[³H]乙酰-D-半乳糖胺用于糖蛋白生物合成的利用情况。
Utilization by the isolated perfused rat liver of N-acetyl-D-[1-14C]galactosamine and N-[3H]acetyl-D-galactosamine for the biosynthesis of glycoproteins.
作者信息
MacNicoll A D, Wusteman F S, Powell G M, Curtis C G
出版信息
Biochem J. 1978 Aug 15;174(2):421-6. doi: 10.1042/bj1740421.
Abstract
The isolated perfused rat liver system has been used to monitor the utilization of N-[3H]acetyl-D-galactosamine and N-acetyl-D-[1-14C]galactosamine for the biosynthesis of radiolabelled glycoproteins, which are subsequently secreted into the plasma. Both radiolabels appear in a number of different glycoproteins, predominantly as sialic acid and N-acetylglucosamine. The ratio of labelled sialic acid to labelled N-acetylglucosamine varies for different glycoproteins, but the bulk of N-acetyl-D-galactosamine is incorporated without deacetylation.
摘要
离体灌注大鼠肝脏系统已被用于监测 N-[3H]乙酰-D-半乳糖胺和 N-乙酰-D-[1-14C]半乳糖胺用于生物合成放射性标记糖蛋白的利用情况，这些糖蛋白随后被分泌到血浆中。两种放射性标记物出现在多种不同的糖蛋白中，主要以唾液酸和 N-乙酰葡糖胺的形式存在。不同糖蛋白中标记唾液酸与标记 N-乙酰葡糖胺的比例各不相同，但大部分 N-乙酰-D-半乳糖胺在未脱乙酰化的情况下被掺入。
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Utilization by the isolated perfused rat liver of N-acetyl-D-[1-14C]galactosamine and N-[3H]acetyl-D-galactosamine for the biosynthesis of glycoproteins.离体灌注大鼠肝脏对 N-乙酰-D-[1-¹⁴C]半乳糖胺和 N-[³H]乙酰-D-半乳糖胺用于糖蛋白生物合成的利用情况。
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引用本文的文献
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2
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Biochem J. 1980 Jan 15;186(1):279-86. doi: 10.1042/bj1860279.
本文引用的文献
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Biochem J. 1968 May;107(5):637-44. doi: 10.1042/bj1070637.
2
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.还原和S-羧甲基化蛋白质的制备及酶促水解
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The degradation of intravenously injected chondroitin 4-sulphate in the rat.大鼠体内静脉注射的硫酸软骨素4-硫酸盐的降解
Biochem J. 1973 Aug;134(4):1009-13. doi: 10.1042/bj1341009.
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Glucosamine metabolism in regenerating rat liver.再生大鼠肝脏中的葡萄糖胺代谢
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Biochem Soc Trans. 1977;5(2):442-3. doi: 10.1042/bst0050442.
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